Generation of genetically engineered CHO cell lines to support the production of a difficult to express therapeutic protein

Introduction Chinese Hamster Ovary (CHO) cells are widely used for the large scale production of recombinant biopharmaceuticals. These cells have been extensively characterised and approved by regulatory authorities for production of biopharmaceuticals. During the last years more and more cell-line engineering strategies have been developed to enhance productivity and quality. CHO cell line engineering work has made remarkable progress in optimizing products or titers by focusing on manipulating single genes and selecting clones with desirable traits. In this work it is shown how cell line engineering approaches enable the expression of a challenging to express “novel therapeutic protein”. The expression of the “novel therapeutic protein” in CHO cells resulted in significant reduced cell growth as well as low productivity.